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KMID : 0368419890320040265
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Journal of Plant Biology
1989 Volume.32 No. 4 p.265 ~ p.274
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Role of Calcium on Auxin-Induced Ethylene Production in Etiolated Mungbean(Vigna radiata W.) Hypocotyls
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Abstract
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The effect of Ca^2+ on auxin-induced ethylene production in etiolated mungbean (Vigna radiata W.) hypocotyls was studied.
Auxin-induced ethylene production by mungbean seedlings which had been germinated in the presence of 5-10mM Ca^2+ (High Ca^2+; HC) is greater than that by seedlings which had been germinated in distilled water (Low Ca^2+; LC). The effect of Ca^2+ on auxin-induced ethylene production was greately increased after 12hr of incubation period. The stimulation of auxin-induced ethylene production by Ca^2+ was specific, since divalent cations, such as Mg^2+ and Mn^2+ did not enhance auxin-induced ethylene production. Calcium also promoted ethylene evolution induced by methionine and 1-Aminocyclopropane-1-carboxylic acid(ACC).
The effect of Ca^2+ on auxin-induced ethylene production was not caused by increase in free IAA or ACC contents of hypocotyl tissue. Dimethyl sulfoxide and Triton X-100, that disrupts the membranes, inhibited ethylene production to a greater extent in LC segments than in HC segments. Addition of Ca^2+ to the incubation medium for LC segments resulted in enhancement of ethylene production probably beacuse the membrane integrity is supported under thesse conditions. Comparison of activity of Ethylene Forming Enzyme(EFE) in LC and HC hypocotyl segments indicated that the enzyme activity of HC was about 2 times higher than that of LC. It is suggested that Ca^2+ increases the activity of plasma membrane-bound EFE through its stabilizing effect on the membrane, which in turn brings about promotion of ethylene prodution.
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